July - September 2015, Volume 11, Issue 3

Wen F, Zhou L, Wu X, Xia S, Sun C, Yang Z

Characterization of mIRNA and mRNA Expression Profiles in Normal and Resistin-Treated Mouse Liver by Microarray

Acta Endo (Buc) 2015, 11 (3): 284-293
doi: 10.4183/aeb.2015.284

Aims. To investigate the changes in the miRNAs and mRNAs expressed in the liver upon induction of “hyperresistinemia”. Methods. We identified mRNA and miRNAs that were differentially expressed between normal and resistin-treated liver tissue using microarrays. Expression was validated using quantitative RT-PCR (qRT-PCR). The putative targets and pathways of the differentially expressed miRNAs and mRNAs were investigated, respectively, using various computational algorithms. In addition, the interactions between differentially expressed miRNAs and mRNAs were analyzed. Results. After the filtration of the signals below the threshold level, we identified 34 miRNAs and 875 genes with expression levels different by more than 1.5-fold and 2.0-fold, respectively, between the two groups. These observations were confirmed by qRT-PCR. Bidirectional prediction analyses showed that the differentially expressed miRNAs may be inversely regulated by their predicted targets. Conclusion. Hyperresistinemia results in changes in the miRNAs and mRNAs expressed in the liver.

Keywords: EMF, oxidative stress, testosterone, testis, F1 generation, prenatal period

Correspondence: Zaiqing Yang MD, Huazhong Agricultural University, College of Life Science and Technology, Wuhan City, Hubei Province, 430070, P.R.China, E-mail: yangzq@mail.hzau.edu.cn