January - March 2017, Volume 13, Issue 1

Gumustekin M, Arici A, Cilaker Micili S, Karaman M, Guneli ME, Tekmen I

HGF/c-Met Pathway has a Role in Testicular Damage in Diabetes Induced by Streptozotocin

Acta Endo (Buc) 2017, 13 (1): 17-22
doi: 10.4183/aeb.2017.17

Objective. The aim of the study was to investigate the role of Hepatocyte Growth Factor (HGF)/c-Met pathway in testicular damage provoked by streptozotocin (STZ)- induced diabetes and the effects of insulin treatment on the HGF/c-Met pathway. Methods. Total 21 paraffin-embedded testicular tissues of control (n=7), streptozotocin (STZ)-induced diabetic (n=7) and insulin-treated diabetic (n=7) Wistar albino rats were used in this study. Testicular damage was examined histologically and by Johnsen’s score was also evaluated. Immunohistochemical stainings of HGF and c-Met were analysed by using antibodies against HGF and c-Met. Results. We found the degeneration in seminiferous tubule epithelium and disorganization of spermatogenetic cell series in testis tissues of diabetic rats. We also determined decrease both in seminiferous tubule diameter and Johnsen’s scores in diabetic group. The expressions of HGF and c-Met in seminiferous tubule epithelium and in spermatogenic cells (especially spermatocytes and spermatids) were significantly increased in diabetic rats compared to those of control. Insulin treatment significantly reduced the diabetes-induced morphological changes and HGF/c-Met over expressions in the diabetic rat testis. Conclusion. HGF/c-Met pathway might have a role in diabetes- induced testicular damage. Drugs acting on this pathway might be effective to prevent or delay the testicular damage induced by diabetes.

Keywords: diabetes, HGF/c-Met, insulin, testis, testicular damage

Correspondence: Mukaddes Gumustekin MD, PhD, Professor of Pharmacology, Dokuz Eylul University School of Medicine, Dept. of Pharmacology, 35340, Narlidere, Izmir, Turkey, E-mail: gumustek@deu.edu.tr