April - June 2014, Volume 10, Issue 2

Yousefian E, Ghaffari Novin M, Kardi M.T, Fadaei Fathabadi F, Mastery Farahani R, Allahveisi A, Alaee S

Levels of mRNAs for Membrane Beta and Gama Progesterone Receptors in the Falopian Tubes of Women with Ectopic Pregnancy

Acta Endo (Buc) 2014, 10 (2): 195-202
doi: 10.4183/aeb.2014.195

Background. The transport of gametes and embryos is facilitated by motile cilia lining the inside of the fallopian tube. Progesterone regulates ciliary beat frequency (CBF) through multiple types of progesterone receptors in the fallopian tube. Membrane progesterone receptors beta and gamma (mPRβ and mPRγ) are both expressed in the ciliated cell lining of the fallopian tubes of humans. This study aimed at exploring the mRNA expression of mPRβ and mPRγ in the fallopian tubes of women with ectopic pregnancy. Materials and Methods. In this Case control study, a quantitative reverse transcriptase polymerase chain reaction was performed to determine the mRNA expression of mPRβ and mPRγ in the fallopian tubes obtained from 12 women with ectopic pregnancies, 12 women with normal pregnancies, and 12 healthy nonpregnant women in the luteal phase of their menstrual cycle. Results. It was indicated that mPRβ and mPRγ were expressed in the fallopian tube of the three groups of participants. However, the expression of mPRβ and mPRγ mRNA in the fallopian tube of women with ectopic pregnancy was not significantly different from that of the nonpregnant and normal pregnant women. Conclusion. These results suggest that mPRs might play no role in etiology of ectopic pregnancies associated with disturbed progesterone signalling.

Keywords: mPRß, mPR?, Fallopian Tube, Ectopic Pregnancy.

Correspondence: Marefat Ghaffari Novin MD, PhD, Cellular and Molecular Biology Research Center, Infertility and Reproductive Health Research Center and Biology and Anatomical Sciences Dept., “Shahid Beheshti” University of Medical Sciences, 1985717443, Tehran, Islamic Republic of Iran, E-mail: mghaffarin@sbmu.ac.ir