July - September 2014, Volume 10, Issue 3

Tsompos C, Panoulis C, Toutouzas K, Zografos G, Papalois A

The Acute Effect of Erythropoietin on Mean Corpuscular Hemoglobin Levels During Ischemia Reperfusion Injury in Rats

Acta Endo (Buc) 2014, 10 (3): 363-372
doi: 10.4183/aeb.2014.363

Background. The aim of this experimental study was to examine the effect of erythropoietin (Epo) on rat model and particularly in an ischemia-reperfusion (IR) protocol. The beneficial effect or non-effectiveness of that molecule were studied haematologically using blood mean corpuscular hemoglobin levels (MCH). Materials and methods. Forty rats of mean weight 247.7 g were used in the study. MCH levels were measured at these time points: on 60 min after reperfusion (groups A and C), and on 120 min after reperfusion (groups B and D). C and D groups were administered by Epo. Results. Epo administration nonsignificantly increased the MCH levels by 0.19 pg [-0.34 pg - 0.72 pg] (P= 0.4762). This finding was in accordance with the results of paired t-test (P= 0.3827). Reperfusion time non-significantly increased the MCH levels by 0.02 pg [-0.51 pg - 0.55 pg] (P= 0.9404), also in accordance with paired t-test (P= 0.9049). However, Epo administration and reperfusion time together produced a nonsignificant combined effect in decreasing the MCH levels by 0.13 pg [-0.18 pg - 0.45 pg] (P= 0.4087). Conclusion. Results of this study indicate that Epo administration, even in short-term prospect of 2 hours reperfusion, starts reversing ischemic damages. These results are also reinforced by predicted MCH values adjusted for weight. However, the significant effect needs more than 2 hours in order to appear.

Keywords: erythropoietin, mean corpuscular hemoglobin levels, reperfusion

Correspondence: Constantinos Tsompos MD, Mesologi County Hospital, Nafpaktou str., Mesologi, 30200, Etoloakarnania, Greece, E-mail: constantinostsompos@yahoo.com