October - December 2014, Volume 10, Issue 4

Gharamaleki H, Parivar K, Soleimani Rad J, Roshangar L, Shariati M

Effects of Electromagnetic Field Exposure During the Prenatal Period on Biomarkers of Oxidative Stress and Pathology of Testis and Testosterone Level of Adult Rats in F1 Generation

Acta Endo (Buc) 2014, 10 (4): 577-587
doi: 10.4183/aeb.2014.577

Objective. The aim of the present study was to evaluate the effect of electromagnetic field (EMF) exposure during developmental period on parameters of oxidative stress and histopathology of testis and testosterone level in adult rat F1 generation. Methods and study design. In treatment group pregnant rats were exposed to 3mT EMF, 50Hz for 21 days. The sham group contained pregnant rats under same condition, but out off the EM field. Pregnant rats in room were used as control group. After delivery, the blood samples of mothers for biochemical analyses of total antioxydant capacity (TAC) and malondialdehide (MDA) were provided. The male pups were kept until maturity, then their TAC, MDA and testosterone levels were analyzed, also their tests were removed for investigation of histopathology changes with light microscopy. Results. Biochemical analysis showed that TAC and MDA was significantly increased in pregnant rats in the treatment group when compared with the control group (p<0.05). In adult male of F1 generation MDA was significantly increased in treatment group, but TAC and the testosterone level was significantly decreased in the treatment group as compared with the control group (p<0.05). Microscopic results revealed that in experimental group seminiferous epithelium contained many small irregular empty spaces as the sign of cellular sloughing, spermatogenic cells appeared to be disrupted. The nuclei of spermatogonia cells were heterochromatic, also dense of germinal epithelium and the number of spermatozoa was decreased. Conclusion. The results of this study suggest that pregnant rats exposure in EMF led to oxidative stress in adult male of F1 generation and showed adverse effect on testosterone and spermatogenesis in adulthood which may produce subfertility.

Keywords: EMF, oxidative stress, testosterone, testis, F1 generation, prenatal period.

Correspondence: Hamideh Gharamaleki MD, Department of Biology, Science and Research Branch, Islamic Azad University, Fars, 985193638115, Islamic Republic of Iran, E-mail: hamideh. gharamaleki@gmail.com