July - September 2015, Volume 11, Issue 3

Borhani N, Ghaffari Novin M, Manoochehri M, Rouzrokh M, Mansouri A, Omrani D

Downregulation of Fibroblast Growth Factor Receptor 2 in Prepuce Tissue of Children with Isolated Hypospadias

Acta Endo (Buc) 2015, 11 (3): 306-311
doi: 10.4183/aeb.2015.306

Context. The androgen/androgen receptor interaction plays a critical role in the development of the male genital system, and investigations around these receptors can expand our knowledge about mechanisms of some male abnormalities such as hypospadias. Objective. In the present study, the expression of androgen receptor (AR), fibroblast growth factor-10 (FGF10), and fibroblast growth factor receptor-2 (FGFR2) genes were investigated in prepuce tissue of healthy and hypospadic children. Design. It was a case-control study with follow-up about up to eight months after operation. Subjects and Methods. Patients’ tissue samples (n=20) and controls tissue samples (n=20) were collected after surgery and circumcision. After RNA extraction and cDNA synthesis, the Real time PCR was performed to evaluate and compare the levels of gene expressions. Both groups were checked until eight months after surgery. Results. The results showed that FGFR2 gene expression had significantly decreased in prepuce tissue of patients compared with healthy children (p-value <0.05). Patients and healthy children follow up showed that 20% of hypospadic patients who needed reoperation had AR gene upregulation along with FGF10 and FGFR2 downregulation. Conclusions. Taken together, interestingly, FGF10/ FGFR2 signaling has an important role in external genital system development and wound healing process.

Keywords: Androgen receptor, FGFR2, Gene expression, Hypospadias, Real time PCR.

Correspondence: Davood Omrani MD, “Shahid Beheshti” University of Medical Sciences, Department of Genetics, School of Medicine, Kudakyar Alley, Daneshjoo Blvd., Yaman Avenue, Chamran Highway, Tehran, Islamic Republic of Iran, E-mail: davood_omrani@sbmu.ac.ir