April - June 2017, Volume 13, Issue 2

Farahmandlou N, Oryan S, Ahmadi R, Eidi A

Association of Testosterone with Colorectal Cancer (HT29), Human Glicoblastoma (A172) and Human Embryonic Kidney (HEK293) Cells Proliferation

Acta Endo (Buc) 2017, 13 (2): 144-149
doi: 10.4183/aeb.2017.144

Testosterone influences cancer development. This in vitro experiment was exerted to determine the association of testosterone with human colorectal cancer(HT29), glioblastoma (A172) and human embryonic kidney(HEK293) cells proliferation. HT-29, A172 and HEK293 cell lines were cultured in standard growth medium, then randomly divided into control group (not exposed to testosterone) and groups exposed to 1, 10, 100 and 1000 μg/mL of testosterone. Cell viability was quantified by MTT assay. Statistical analysis was performed using ANOVA. Viability of HEK293 cells significantly increased in groups exposed to 1 μg/mL and decreased in groups exposed to 100 and 1000 μg/mL of testosterone compared to control group (P<0.05, P<0.05 and P<0.001, respectively). Viability of HT29 cells significantly increased in groups exposed to 10 and 100 μg/mL of testosterone and significantly decreased when exposed to 1000 μg/mL of testosterone compared to control group (P<0.05, P<0.001 and P<0.001, respectively). Viability of A172 cells significantly decreased in groups exposed to 100 and 1000 μg/mL of testosterone compared to control group (P<0.001). In conclusion, different doses of testosterone have enhancing or suppressive effects on HEK293, HT29 and A172 cells proliferation; according to which, considering clinical use of testosterone therapy for cancer treatment is a highly controversial issue.

Keywords: Testosterone, HT-29, A172, HEK 293, Proliferation

Correspondence: Rahim Ahmadi MD, Islamic Azad University, Hamedan Branch, Dept. of Biology, Hamedan, 65155, Islamic Republic of Iran, E-mail: rahahmadi2001@yahoo.com