January - March 2017, Volume 13, Issue 1

Wen F, Yang Y, Sun C, Fang H, Nie L, Li L, Liu Y, Yang Z

Resistin Inhibits Glucose-Stimulated Insulin Secretion through miR-494 by Target on STXBP5

Acta Endo (Buc) 2017, 13 (1): 32-39
doi: 10.4183/aeb.2017.32

Aims. Resistin has been reported to impair the pancreatic beta cells and associated with insulin resistance. MicroRNAs (miRNAs) are short, endogenously produced non-coding ribonucleotides that bind mRNAs and function mainly as negative regulators in mammals. MiRNAs have been implicated in many diseases, including insulin resistance and diabetes. A considerable body of evidence has indicated an important function for miRNAs in insulin secretion. The current study was designed to investigate the effects of miR-494 in the reductions in insulin secretion attributable to resistin. Methods. Insulin secretion was determined by ELISA, and expressions of genes were identified using quantitative RT-PCR (qRT-PCR) or Western blot analysis. Results. Insulin secretion was significantly reduced by resistin. Overexpression of miR-494 inhibited insulin secretion both in diet culture and high glucose medium in MIN6 cell lines. MiR-494 down-regulated the protein level of STXBP5 by pairing with sites in the 3′UTR. Conclusion. miR-494 is involved in the insulin secretion regulated by resistin via its effects on STXBP5 in MIN6 cells.

Keywords: resistin, insulin secretion, miR-494, STXBP5

Correspondence: Zaiqing Yang PhD, Huazhong Agricultural University, College of Life Science and Technology, Wuhan City, Hubei Province, 430070, P.R.China, E-mail: yangzq@mail.hzau.edu.cn