July - September 2019, Volume 15, Issue 3

Shakeri H, Khoshi A, Kaffash Bajestan M, Farahi A, Javadzadeh MS, Hosseini Z, Mohammadi R

Association of Irs1 GLY971ARG Gene Polymorphism With Insulin Resistance in Iranian Newly Diagnosed Diabetic Adults

Acta Endo (Buc) 2019, 15 (3): 317-322
doi: 10.4183/aeb.2019.317

important role in insulin signaling and the common Gly971Arg polymorphism is related to type 2 diabetes (T2D). IRS-1 Gly971Arg polymorphism can modify tyrosine phosphorylation at a specific site of IRS-1 and may have a critical role in the development of insulin resistance (IR). Objective. The purpose of this study was to investigate the association between this polymorphism and IR in Iranian patients with newly-diagnosed type 2 diabetes. Design. The study was conducted on 114 individuals with newly-diagnosed T2D and 118 healthy matched controls, aged 20-80 years. Fasting blood glucose and insulin were measured by the enzymatic method and enzyme-linked immunosorbent assay, respectively. Insulin-resistance was calculated by homeostasis model assessment estimatedinsulin resistance (HOMA-IR). The gene polymorphism was examined by polymerase chain reaction-restriction fragment length polymorphism. Results. There are significant differences between IRS1 Gly971Arg polymorphism and studied individuals (P<0.0001). The findings showed that the risk of developing T2D in individuals who had R-alleles was 3.74 folds higher than those without R-alleles. However, IRS1 Gly971Arg polymorphism was not associated with high HOMA-IR, high BMI and familial history of diabetes. Conclusions. Even though there was not a significant relationship between IRS-1 G971R polymorphism with insulin resistance and high BMI, this polymorphism was correlated to newly-diagnosed diabetic patients. Thus, the evaluation of IRS-1 G971R polymorphism may be helpful for predicting T2D new cases.

Keywords: type 2 diabetes, insulin resistance, IRS-1, gene polymorphism.

Correspondence: Amirhosein Khoshi, Clinical Biochemistry Ph.D., North Khorasan University of Medical Sciences, Clinical Biochemistry, Department of School of Medicine, Bojnurd 9417694735, Iran, E-mail: ahkh83@gmail.com