January - March 2022, Volume 18, Issue 1

Soyman Z, Durmus S, Ates S, Simsek G, Sozer V, Kundaktepe BP, Kurtulus D, Gelisgen R, Sal V, Uzun H

Circulating MIR-132, MIR-146A, MIR-222, AND MIR-320 Expression in Differential Diagnosis of Women with Polycystic Ovary Syndrome

Acta Endo (Buc) 2022, 18 (1): 13-19
doi: 10.4183/aeb.2022.13

Purpose. The aim of the study was to investigate whether the circulating miR-132, miR-146a, miR-222, and miR-320 levels are used in the differential diagnosis of women with polycystic ovary syndrome (PCOS) and healthy women. Methods. This prospective case-control study included 50 women with PCOS and age- and body mass index- matched 50 healthy controls. The hormone and lipid profiles, levels of microRNAs (miRNAs), and parameters of carbohydrate metabolism were measured. Results. Expression levels of miRNAs were assessed using the two-step quantitative real-time polymerase chain reaction. Circulating miR-132, miR-146a and miR- 222 levels were significantly downregulated in the PCOS group compared with the control group. The miR-320 levels did not differ between the two groups. Free testosterone was negatively correlated with miR-132, miR-146a and miR-222. Insulin was negatively correlated with miR-132 and miR-146a. Conclusions. The results of the study revealed that miRNA expression, may suggest a possible distinction between healthy women and PCOS patients. miR-132, miR-146a, and miR-222 may have key functions in the pathogenesis of PCOS.

Keywords: Polycystic ovary syndrome, miR-132, miR-146a, miR-222.

Correspondence: Prof. Dr. Hafize Uzun, Istanbul Atlas University, Department of Medical Biochemistry, Faculty of Medicine, Istanbul, Turkey, E-mail: huzun59@hotmail.com