The International Journal of Romanian Society of Endocrinology / Registered in 1938

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October - December 2022, Volume 18, Issue 4
Endocrine Care

Demiral M, Celebi HBG, Cander S, Yerci O, Eren E, Demirbilek H

Two Opposite Phenotypes of Glucose Disorders in a Family with Heterozygous P.SER453LEU (C.1358C> T) Mutation in the Glucokinase (GCK) Gene: Maturity Onset Diabetes in Young and Insulinoma

Acta Endo (Buc) 2022, 18 (4): 458-465
doi: 10.4183/aeb.2022.458

Background. Heterozygous gain-of-function mutations in the glucokinase (GCK) gene cause hyperinsulinaemic hypoglycaemia (GCK-HI), while lossof- function mutations lead to a monogenic type of diabetes (GCK-MODY). We, herein, report a heterozygous GCK gene mutation in a large family with GCK-MODY and insulinoma in one individual from the same family. Patients and methods. The proband, an 11-yearold male, was referred for asymptomatic mild hyperglycemia (fasting glucose:121 mg/dL) and HbA1c of 6.1%. Segregation analysis of the family revealed multiplex members with asymptomatic fasting hyperglycaemia or non-insulindependent diabetes and 33-year-old maternal uncle of the proband case had a history of distal pancreatectomy due to the diagnosis of insulinoma. His preoperative investigations were revealed fasting glucose of 31 mg/dL, insulin: 7μU/ mL, C-peptide: 2.6 mg/dL, and a low HbA1c(4.0%) which was suggestive for recurring hypoglycaemia episodes. Postpancreatectomy he developed mild fasting hyperglycemia (115-136 mg/dL). Results. Genetic analysis revealed heterozygous p.Ser453Leu(c.1358C> T) mutation in the GCK gene in the proband. In segregation analysis, the identical heterozygous p.Ser453Leu(c.1358C> T) GCK gene mutation was detected in all of the other affected family members for whom a DNA analysis was applicable. The maternal uncle was first diagnosed with insulinoma and underwent a pancreatectomy. He also had an identical mutation in a heterozygous state. Conclusion. We, to the best of our knowledge, firstly identified these two entirely distinct phenotypes of glucose metabolism, GCK-MODY and GCK-HI, due to an identical heterozygous p.Ser453Leu (c.1358C> T) mutation in the GCK. Further studies required to elucidate this new phenomenon and understanding the genotype-phenotype relationship of GCK gene mutations.

Keywords: GCK gene, MODY, insulinoma, hyperinsulinaemic hypoglycaemia.

Correspondence: Meliha Demiral MD, Balikesir Atatürk City Hospital, Department of Paediatric Endocrinology, Balikesir, Turkey, E-mail: