ACTA ENDOCRINOLOGICA (BUC)

Objective. Infertility affects an estimated 17.5% of couples worldwide, according to the last report published in 2023 by WHO. A significant cause of this global fertility decline is due to environmental endocrine-disrupting chemicals exposure, which have the ability to interfere with the endocrine system. Bisphenol A (BPA), a synthetic chemical product leads to health concerns for its endocrinedisrupting properties. Jujube honey contains antioxidants and active compounds, has various therapeutic properties. This study aims to determine testicular dysfunction and endocrine imbalance induced by BPA chronic exposure and the possible protective effect of jujube honey in gerbil. Materials and methods. Eighteen adult male gerbil was either considered as control (n=6, group 1) or exposed to 50 mg BPA/kg/day Group 2, n=6) and the BPAtreated group was given jujube honey at 200 mg/kg/day by oral gavage Group 3, n=6) for six weeks. At the end of the experiment, testicular weight, epididymal sperm count, endocrine status, histopathology of testis tissue and oxidative stress parameters were evaluated. Results. The results showed a decrease in testicular weight, sperm quality and plasma levels of testosterone and estradiol in the BPA-treated group. Furthermore, histopathological findings revealed germinal epithelium alteration characterized by germ cell degeneration, disordered arrangement of the germ cell layers and vacuolization and decreased number of Ki-67-positive germ cells. The results showed a decrease in testicular levels of superoxide dismutase, catalase and reduced glutathione with an increase of malondialdehyde in the BPA-treated group. However, in the jujube honey+BPA group, histopathological studies revealed a systematic array of germ cells with less vacuolization, improved sperm parameters, restored plasma testosterone and estradiol levels and increased antioxidative capacity of the testes. Conclusion. These findings suggest a potential protective effect of jujube honey against BPA-induced testicular damage and oxidative stress caused by endocrinedisrupting chemicals.