ACTA ENDOCRINOLOGICA (BUC)

The International Journal of Romanian Society of Endocrinology / Registered in 1938

in Web of Science Master Journal List

Acta Endocrinologica(Bucharest) is live in PubMed Central

Year Volume Issue First page
10.4183/aeb.
Author
Title
Abstract/Title
From through

  • General Endocrinology

    Leonte L, Coculescu M, Radian S, Fica S, Caragheorgheopol A, Marinescu B, Bohaltea LC, Grigorescu F

    Anti-Mullerian hormone (AMH) as a useful marker in diagnosis of polycystic ovary syndrome

    Acta Endo (Buc) 2007 3(1): 1-12 doi: 10.4183/aeb.2007.1

    Abstract
    The mechanism underlying anovulation in the polycystic ovary syndrome (PCOS) remains unclear, although an excessive number of small antral follicles at ultrasound scans and discrepancies with selected follicles sustain the hypothesis of altered follicular development. Anti-M?llerian (AMH) hormone is a member of TGF-b super family of growth factors produced by granulosa cells of pre- and small-antral follicle. The 2 to 3 fold increase in the number of growing follicles in the ovary from PCOS women is reflected by an increase in serum concentration of AMH and thus, this hormone may be a good marker of PCOS.\r\nAim. This study was intended to implement ultra-sensitive ELISA measurement of serum AMH from PCOS women and search for a potential correlation with clinical and laboratory parameters.\r\nSubjects and methods. Sera from patients with PCOS (n = 42) and control women (n = 22) were used for ELISA measurement of AMH (AMH-EIA, Beckman Coulter) with sensitivity of 0.7 pmol/L.\r\nResults. We found a serum concentration of AMH almost 3 folds higher in patients with PCOS compared to controls (73.7 ? 7.5 vs. 25.7 ? 3.9 pmol/L, P < 0.0001). Differences were even higher in lean subjects. A positive correlation was found between total testosterone and LH levels, but not with serum FSH or insulin. Moreover, AMH concentration was correlated to more hyperandrogenic PCOS and with amenorrhea, and thus to the severity of the syndrome.\r\nConclusion. Measurement of serum AMH may be used as a valuable marker for PCOS to confirm diagnosis and evaluate the extent of follicular dysfunction in relation with hyperandrogenism and menstrual disturbances.
  • General Endocrinology

    Radian S, Bensaada M, Lautier C, Moles JP, Grigorescu F, Gussi I, Badiu C, Nastasia S, Hudita D, Leonte L, Marinescu B, Coculescu M

    Molecular genetics strategies to identify vasotocin coding sequences in humans: family-specific approach using genomic DNA and fetal tissues mRNAs

    Acta Endo (Buc) 2005 1(2): 131-144 doi: 10.4183/aeb.2005.131

    Abstract References
    Vertebrate nonapeptide neurohormones constitute an evolutionarily conserved family, involved in vital functions, such as hydro-osmotic balance regulation, reproduction and social behaviour. Two human members of this family are known, vasopressin (AVP) and oxytocin (OXT), with their highly homologous genes closely located on Chr 20p13. Presence of vasotocin (AVT) in man has been suggested, but remains controversial, and genetic evidence is lacking. AVT activity could be explained by the presence of a third distinct gene for AVT or an RNA-processing mechanism involving products of AVP and/or OXT genes. To test the first hypothesis, we developed bioinformatics and experimental approaches using genomic DNA and fetal tissues mRNAs. Family-specific primers for AVT and neurophysin were designed based on CODEHOP strategy and used in our experiments. Results of bioinformatics and genomic DNA experiments (family-specific and Alu step-out PCR) suggest there is no evidence for an AVT gene in the genome. RNA-based techniques 3?-RACE and Family-Specific Domain Restriction Fragment RTPCR provided evidence for new transcript species that could code for AVT. Further experiments will be needed to characterize them. We discuss potential mechanisms of AVT mRNA generation based on AVP and OXT mRNAs, by alternative splicing, heterologous transsplicing or RNA-editing. While all methods we developed proved feasible, current results suggest there is no AVT gene in the genome, but specific mRNAs could be present in fetal tissues. Their full characterization may potentially allow identification of vasotocin mRNA and shed light on a subject of fundamental scientific interest.
    1. Dale HH. Evidence concerning the endocrine function of the neurohypophysis and its nervous control. In: The Neurohypophysis, editor Heller HH. London: Butterworth?s, 1957, 1-9.
    2. Dantzer R. Vasopressin, gonadal steroids and social recognition. Prog Brain Res. 1998;119:409-14.
    3. Bielsky IF, Young LJ. Oxytocin, vasopressin, and social recognition in mammals. Peptides. 2004;25(9):1565-74. [CrossRef]
    4. Kosfeld M, Heinrichs M, Zak PJ, Fischbacher U, Fehr E. Oxytocin increases trust in humans. Nature. 2005;435(7042):673-6. [CrossRef]
    5. Hoyle CH. Neuropeptide families and their receptors: evolutionary perspectives. Brain Res. 1999;848(1-2):1-25. [CrossRef]
    6. Sausville E, Carney D, Battey J.The human vasopressin gene is linked to the oxytocin gene and is selectively expressed in a cultured lung cancer cell line. J Biol Chem. 1985;260(18):10236-41.
    7. Pavel, S. Arginine vasotocin as a pineal hormone. J. Neural. Transmission 1978; 13:135-155.
    8. Coculescu M, Pavel S. Arginine vasotocin-like activity of cerebrospinal fluid in diabetes insipidus. J Clin Endocrinol Metab. 1973;36(5):1031-2. [CrossRef]
    9. Catrina SB, Coculescu M, Andersson M. A chemical method to isolate hypothalamic nonapeptides by coupling cyst(e)in with bimane. J Cell Mol Med. 2001;5(2):195-7. [CrossRef]
    10. Badiu C, Coculescu M, Moller M. Arginine vasotocin mRNA revealed by in situ hybridization in bovine pineal gland cells. Cell Tissue Res. 1999;295(2):225-9. [CrossRef]
    11. Ervin MG, Amico JA, Leake RD, Ross MG, Robinson AG, Fisher DA. Arginine vasotocin and a novel oxytocin-vasotocin-like material in plasma of human newborns. Biol Neonate. 1988;53(1):17-22. [CrossRef]
    12. Pavel S. Evidence for the ependymal origin of arginine vasotocin in the bovine pineal gland. Endocrinology. 1971;89(2):613-4. [CrossRef]
    13. Pavel S, Dorcescu M, Petrescu-Holban R, Ghinea E. Biosynthesis of a vasotocin-like peptide in cell cultures from pineal glands of human fetuses. Science. 1973;181(106):1252-3. [CrossRef]
    14. Rose TM, Schultz ER, Henikoff JG, Pietrokovski S, McCallum CM, Henikoff S. Consensusdegenerate hybrid oligonucleotide primers for amplification of distantly related sequences. Nucleic Acids Res. 1998;26(7):1628-35. [CrossRef]
    15. <http://blocks.fhcrc.org/blocks/codehop.html>
    16. Fuentes JJ, Pucharcos C, Pritchard M, Estivill X. Alu-splice PCR: a simple method to isolate exoncontaining fragments from cloned human genomic DNA. Hum Genet. 1997;101(3):346-50. [CrossRef]
    17. Siebert PD, Chenchik A, Kellogg DE, Lukyanov KA, Lukyanov SA. An improved PCR method for walking in uncloned genomic DNA. Nucleic Acids Res. 1995;23(6):1087 [CrossRef]
    18. Matz M, Shagin D, Bogdanova E, Britanova O, Lukyanov S, Diatchenko L, Chenchik A. Amplification of cDNA ends based on template-switching effect and step-out PCR. Nucleic Acids Res. 1999;27(6):1558-60. [CrossRef]
    19. Michel G, Levy B, Chauvet MT, Chauvet J, Acher R. Complete amino acid sequence of goose VLDVneurophysin. Traces of a putative gene conversion between promesotocin and provasotocin genes. Int J Pept Protein Res. 1990;36(5):457-64. [CrossRef]
    20. Ruppert S, Scherer G, Schutz G. Recent gene conversion involving bovine vasopressin and oxytocin precursor genes suggested by nucleotide sequence. Nature. 1984 Apr 5-11;308(5959):554-7. [CrossRef]
    21. Pavel S. Evidence for the presence of lysine vasotocin in the pig pineal gland. Endocrinology. 1965 ;77(5):812-7. [CrossRef]
    22. Pavel S. Pineal vasotocin and sleep. Environmental Physiology. Vol. 18 in Advances in Physiological Sciences. Proceedings of the 28th International Congress of Physiological Sciences, Budapest, 1980. Obal, F., and G. Benedek, Eds. Budapest: Akademiai Ki
    23. Coculescu M, Serbanescu A, Temeli E. Influence of arginine vasotocin administration on nocturnal sleep of human subjects. Waking Sleeping. 1979;3(3):273-7.
    24. Mihai R, Coculescu M, Wakerley JB, Ingram CD. The effects of [Arg8]vasopressin and [Arg8]vasotocin on the firing rate of suprachiasmatic neurons in vitro. Neuroscience. 1994 ;62(3):783-92. [CrossRef]
    25. Lefebvre DL, Zingg HH. Novel vasopressin gene-related transcripts in rat testis. Mol Endocrinol. 1991;5(5):645. [CrossRef]
    26. Foo NC, Funkhouser JM, Carter DA, Murphy D. A testis-specific promoter in the rat vasopressin gene. J Biol Chem. 1994;269(1):65.
    27. Caudevilla C, Serra D, Miliar A, Codony C, Asins G, Bach M, Hegardt FG. Natural trans-splicing in carnitine octanoyltransferase pre-mRNAs in rat liver. Proc Natl Acad Sci U S A. 1998;95(21):12185-90. [CrossRef]
    28. Finta C, Zaphiropoulos PG. Intergenic mRNA molecules resulting from trans-splicing. J Biol Chem. 2002;277(8):5882-90. Epub 2001 Nov 28. [CrossRef]
    29. Mohr E, Peters A, Morris JF, Richter D. Somatic nonhomologous crossing-over between neuropeptide genes in rat hypothalamic neurons. Proc Natl Acad Sci U S A. 1994;91(24):11403-7. [CrossRef]
    30. Eisenberg E, Nemzer S, Kinar Y, Sorek R, Rechavi G, Levanon EY. Is abundant A-to-I RNA editing primate-specific? Trends Genet. 2005;21(2):77-81. [CrossRef]
    31. Levanon EY, Eisenberg E, Yelin R, Nemzer S, Hallegger M, Shemesh R, Fligelman ZY, Shoshan A, Pollock SR, Sztybel D, Olshansky M, Rechavi G, Jantsch MF. Systematic identification of abundant Ato- I editing sites in the human transcriptome. Nat Biotechnol [CrossRef]
  • Book Review

    Dordea Leonte LE

    Fetology. Diagnosis and Management of the Fetal Patient

    Acta Endo (Buc) 2013 9(4): 657-657 doi: 10.4183/aeb.2013.657