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Romanian Academy
The Publishing House of the Romanian Academy
ACTA ENDOCRINOLOGICA (BUC)
The International Journal of Romanian Society of Endocrinology / Registered in 1938in Web of Science Master Journal List
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Acta Endocrinologica (Buc)
Saleh N, Elayan HE, Zihlif M
The Effect of Salbutamol on Pgc-1 a and Glut4 Mrna Expression in the Liver and Muscle of Elderly Diabetic Mice
Acta Endo (Buc) 2018, 14 (2): 184-191doi: 10.4183/aeb.2018.184
Peroxisome proliferator-activated
receptor gamma coactivator 1-alpha (PGC-1α) plays
an important role in the regulation of cellular energy
metabolism, and it is involved in obesity and type 2 diabetes
mellitus (T2DM). Its expression is elevated in the liver of
T2DM mouse models. Literature reports show that chronic
β2 stimulation improved insulin sensitivity in T2DM.
Objectives. We aimed to test the hypotheses that
chronic β2 stimulation-induced improvement in insulin
sensitivity involves changes in the expression of PGC-1α
and glucose transporter 4 (GLUT4).
Animals and Methods. We fed a locally inbred, 8
months old mice, a high fat diet (HFD) to induce diabetes.
These mice gained weight and became insulin resistant. The
β2 agonist salbutamol had a beneficial effect on both glucose
tolerance and insulin sensitivity after 4 weeks.
Results. Salbutamol beneficial effect persisted after
4 weeks of its discontinuation. HFD caused an up regulation
of the hepatic PGC-1 α expression by 5.23 folds (P< 0.041)
and salbutamol reversed this effect and caused a down
regulation by 30.3 folds (P< 0.0001). PGC-1 α and GLUT4
expression in the muscle was not affected by salbutamol (P>
0.05).
Conclusion. Down regulation of the liver’s PGC-
1 α contributes to the beneficial effect of the chronic β2
stimulation on glucose tolerance and insulin sensitivity in
T2DM mice.
Keywords: diabetic mice, salbutamol, PGC-1 a
Correspondence: Malek Zihlif MD, University of Jordan, Faculty of Medicine, Department of Pharmacology, Amman, 11942, Jordan, E-mail: m.zihlif@ju.edu.jo