The International Journal of Romanian Society of Endocrinology / Registered in 1938

in Web of Science Master Journal List

Acta Endocrinologica(Bucharest) is live in PubMed Central

Journal Impact Factor - click here.

Year Volume Issue First page
From through

  • General Endocrinology

    Bandyopadhyay R, Chakraborty S

    Adrenal corticoids induce pineal gland stimulation associated with adrenocortical inhibition of karyomorphology cell proliferation and hormone milieu in male mice (mus musculus)

    Acta Endo (Buc) 2010 6(1): 1-14 doi: 10.4183/aeb.2010.1

    Background. Pineal and adrenocortical cell morphology, dynamics, hormonal analysis and function in response to both natural and synthetic corticoids awaits in depth investigation in mammals. Aim. To investigate the pineal responsiveness to corticoid treatment from combined morphological and hormonal studies in postpubertal male mice. Material and methods. Three groups, each with 14 mice were used as control (C) or treated with the natural corticoid, hydrocortisone (HYC) at a dose of 4 mg/100 g.b.w. and synthetic corticoid, dexamethasone (DEX) at a dose of 4 mg/100 g.b.w. for ten consecutive days. Results. The treatment induced inverse changes in pineal-adrenocortical karyomorphology, cell proliferation (mitotic percentage M%) and hormonal milieu. Whereas both these corticoids caused pineal stimulation as evidenced from significantly increased nuclear diameter (μm) values (C 3.35 ± 0.05, HYC 4.77 ± 0.02, DEX 4.59 ± 0.04, p<0.001) and cell proliferation (M%) (C 1.11 ± 0.09, HYC 1.59 ± 0.07, DEX 1.44 ± 0.05, p<0.01), the changes induced in adrenocortical nuclear diameter in all the zones (p<0.001), cell proliferation (M%) (C 1.38 ± 0.05, HYC 0.53 ± 0.06, DEX 0.70 ± 0.05, p<0.001) and decreased content of adrenal corticosterone (C 0.24 ± 0.03, HYC 0.13 ± 0.01, p<0.001 DEX 0.15 ± 0.02, p<0.01) were those of adrenocortical inhibition. Conclusion. There exists an inverse relationship between the pineal and adrenocortical functions in post pubertal male mice (Mus musculus).
  • General Endocrinology

    Sinha BR, Chakraborty S

    Pineal-thyroid karyomarphology and function in resonse to diversely altered serum T4 levels in rat (rattus rattus)

    Acta Endo (Buc) 2010 6(2): 151-163 doi: 10.4183/aeb.2010.151

    Background. The pineal cytomorphological responsiveness to altered serum T4 levels awaits any comprehensive investigations in mammals .\r\nAim. The aim of the present investigation was to use rats with diversely altered serum T4 level, to study the pineal karyomorphology and functions.\r\nMaterials and Methods. Five groups each with 11 rats were used as controls and T4 (50&#956;g/100g b.w.) for seven consecutive days, thyroidectomized and kept for thirty days and additionally such TX rats, treated with T4 (50&#956;g/100g b.w.) for seven days, were used for pineal karyometry and serum T4 analysis.\r\nResults. They indicated that thyroxine administration evoked hyperactive changes in pineal gland cytomorphology along with enhanced serum T4, as evidenced from increased\r\npinealocyte nuclear diameter (&#956;m) (C 4.71+0.03,T4 5.14+0.04,p<0.001) and decreased nuclear density (C 179.44+4.78,T4 126+4.36,p<0.001) and enhanced serum T4 level\r\n(&#956;g/dL), (C 3.60+0.13,T4 13.40+1.75,p<0.001). Contrarily, thyroidectomized (TX) rats with undetectable T4 levels (<0.05 &#956;g/dL) showed pineal inhibition, as seen from significantly decreased pinealocyte nuclear diameter (&#956;m) (C 4.71+0.03,Tx 4.01+0.04,p<0.001) values, and an increased nuclear density per microscopic field (C179.44+4.78, Tx 208.8+4.47, p<0.005). However, thyroidectomized animals, supplemented with thyroxine (Tx + T4), induced pineal activation as seen from increased pinealocyte nuclear diameter, &#956;m (C 4.71+0.03, Tx + T4 5.26+0.05, p<0.001) associated with increased serum T4 level &#956;g/DL (C 3.60+0.13, Tx+T4 10.92+0.13m p<0.001).\r\nConclusion. The present study argues for a direct pineal-thyroid relationship as interpreted from cytomorphological level and hormone profiles in male albino rats.
  • General Endocrinology

    Das P, Saha I, Chatterjee A, Pramanick K, Chakraborty SB, Maity A, Bhowal S, Pradhan D, Mukherjee D, Maiti BR

    Participation of Phosphatidylinositol-3 Kinase Signalling in Human Chorionic Gonadotropin, Bovine Insulin (B-Insulin) and Human-Insulin-Like Growth Factor-I Induced Oocyte Maturation and Steroidogenesis in the Grey Mullet, Mugil Cephalus

    Acta Endo (Buc) 2020 16(4): 426-436 doi: 10.4183/aeb.2020.426

    Context. The grey mullet, Mugil cephalus, is an edible fish of high economic importance. Breeding biology with reference to hormonal/growth factor regulation of oocyte maturation needs to be known for its commercial production. Objective. The present study was conducted to examine the potency of maturation inducing hormones, chorionic gonadotropin (hCG), bovine-insulin, and insulin like growth factor1 (h-IGF-1) I on ovarian steroidogenesis and oocyte maturation. Design. The role of hormones and growth factors on steroidogenesis and oocyte maturation was investigated using specific inhibitors, Wortmannin for phosphatidylinositol-3 (PI3) kinase, trilostane for 3β-hydroxysteroid dehydrogenase, 1-octanol and 1-heptanol for gap junctions, actinomycin D for transcription and cycloheximide for translation of signal molecules. Methods. Actions of hormonal and growth factors were examined for steroidogenesis, by radioimmunoassay and oocyte maturation by germinal vesicle breakdown (GVBD). Specific inhibitors were used to determine the cell signaling pathways, PI3 kinase. Results. All the inhibitors attenuated the hCGinduced oocyte maturation (GVBD%), steroidogenesis including transcription, translation, gap junctions and PI3 kinase signaling. These inhibitors failed to inhibit h-IGF-I and b-insulin-induced oocyte maturation, steroidogenesis, translation and PI3 kinase signaling. Conclusion. hCG induces oocyte maturation via steroid dependent pathway involving gap junctions, transcription, translation and PI3 kinase signaling, unlike h-IGF-I and b-insulin in the mullet.