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ACTA ENDOCRINOLOGICA (BUC)
The International Journal of Romanian Society of Endocrinology / Registered in 1938in Web of Science Master Journal List
Acta Endocrinologica(Bucharest) is live in PubMed Central
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General Endocrinology
Ustun F, Ustabasioglu FE, Tokuc B, Yimaz Bulbul, Celik M, Ayturk S
Paget's Disease of the Bone Found Incidentally on F-18 FDG PET/CT: Clinical Significance and Differential Diagnostic CriteriaActa Endo (Buc) 2023 19(3): 292-300 doi: 10.4183/aeb.2023.292
AbstractBackground. Paget Disease (PD) is usually asymptomatic and discovered incidentally, it is known that it is exhibited low to high grade increased F-18 FDG uptake. Aim. In this study, we investigated the distinguishability of FDG PET/CT in incidental PD cases from other bone diseases and at different stages of the disease. Patients and Methods. In this cross-sectional, descriptive study, “Paget” identification associated with PET/ CT reports was found in 69 of 18,119 studies (~3.8%). Of the 45 patients (33 males and 12 females) eligible for inclusion in the study, 35.6% had monostotic and 64.4% had polyostotic disease (p>0.5). There was no statistically significant difference in biochemical parameters between groups. Results. According to the radiological appearance of the patients, 36 were in the mixed stage and 9 were in the blastic stage. Only the difference in ALP and creatinine values between the groups was statistically significant. SUVmax, SUVmean and HU values were found to be statistically significantly higher in pagetoid bones compared to control bone lesions. For SUVmax for PD bone lesion we found the 2.55 cutoff point with a sensitivity of 91% and a specificity of 84%. Conclusion. The specific radiological appearance of bone lesions and the evaluation of metabolic activity compared to normal bone seem to help differentiate PD from other lesions. Prospective studies are needed in the differentiation of FDG's disease stage and treatment response evaluation. The ability to differentiate between benign and malignant FDG avid bone lesions in oncological patients’ enables appropriate patient management, including avoiding unnecessary additional invasive procedures such as bone biopsy. -
General Endocrinology
Vladoiu S, Botezatu A, Anton G, Manda D, Paun DL, Oros S, Rosca R, Dinu Draganescu D
The Involvement of VDR Promoter Methylation, CDX-2 VDR Polymorphism and Vitamin D Levels in Male InfertilityActa Endo (Buc) 2017 13(3): 294-301 doi: 10.4183/aeb.2017.294
AbstractIntroduction. Vitamin D (VD) levels were correlated with different health conditions, including reproductive disorders in males. Vitamin D action is mediated through vitamin D receptor (VDR), which acts as a transcription factor. VDR gene promoter is embedded in a GC-rich island. The VDR gene has been shown to have several polymorphisms that affect the receptor function. Aim. To examine the relationship between Cdx- 2 polymorphism (rs17883968), the methylation status of VDR’s promoter and serum levels of 25-hydroxyvitamin D in male infertility. Patients and Methods. A total of 69 infertile men and 37 age-matched controls were enrolled in this study. Vitamin D level assessments were detected using the electrochemiluminescent method. Cdx-2 VDR polymorphism identification was performed by PCR on DNA samples from blood, followed by restriction. Methylation of VDR gene promoter was assessed by qMS-PCR using bisulfite-treated DNA from fresh sperm. Results. Vitamin D levels was found to be significantly decreased in infertile groups compared the controls (p=0.0279). The GG genotype was found in a higher percentage in controls and the AA genotype was higher in infertile group (p=0.0056). Infertile homozygote (GG) and heterozygote (GA) individuals had significantly higher vitamin D levels than AA homozygote. Methylation is higher in individuals with lower vitamin D levels and AA genotype is characterized by higher methylation values. Conclusion. The results provide new insights of Cdx-2 polymorphism is involved in vitamin D deficiency, highlighting the important role of epigenetic modification of vitamin D receptor and male infertility along with the genetic context. -
Case Report
Erem C, Ucuncu O, Nuhoglu I, Turkyilmaz S, Yildiz K, Civan N, Akcay M
Large Adrenocortical Oncocytoma with Uncertain Malignant Potential: Report of a New Case and Review of the LiteratureActa Endo (Buc) 2012 8(2): 295-306 doi: 10.4183/aeb.2012.295
AbstractBackground. Adrenocortical oncocytoma (ACO) is exceedingly rare. To date, only 81 cases are reported in the English literature. Most of ACOs are nonfunctioning and benign.\r\nCase report. We describe a case of ACO incidentally diagnosed in a 54-yearold male patient. Physical examination, routine laboratory studies and hormonal tests were within normal ranges. Abdominal computed tomography (CT) and magnetic resonance imaging showed a large and\r\nheterogeneous tumor (9x7x6 cm) in the left adrenal gland with borderline malignant characteristics. Left adrenalectomy was performed for treatment purposes. The cut\r\nsurface of the resected tumor was heterogeneous with tan brown color with areas of extensive hemorrhage and necrosis.\r\nMicroscopically, the tumor consisted predominantly of large polygonal cells containing eosinophilic granular cytoplasm\r\narranged in a solid pattern with abundant hemorrhage and necrosis. The tumor showed a compressed remnant of adrenal\r\ncortex in the outer the capsule of the mass. No vascular and capsular invasion was noted, and mitotic figures were not\r\nconspicuous. Immunohistochemically, the tumor cells were diffusely and strongly positive for melan-A, vimentin, alphainhibin, weakly positive for synaptophysin and calretinin. The tumor was focal and erratively positive for pancytokeratin. No immunoreactivity was observed form\r\nchromogranin-A, CD10 or p53. The histological diagnosis was ACO with uncertain malignant potential.\r\nConclusions. ACO occurs rarely in adults and preoperative diagnosis is difficult, especially in asymptomatic cases.\r\nIt needs careful evaluation and surgical treatment. According to our knowledge, this is the 2th case of ACO in an adult patient from Turkey in English literature. We\r\ndiscuss this case and review the literature on this unusual entity. -
General Endocrinology
Zengin FH, Karabudak E, Omma T, Culha C
The Relationship between Potential Diet Inflammatory Load and Chemerin, Androgens and Insulin Resistance in Women with Polycystic Ovary SyndromeActa Endo (Buc) 2024 20(3): 295-303 doi: 10.4183/aeb.2024.295
AbstractBackground. In recent years, the effect of inflammation on the pathophysiology of polycystic ovary syndrome (PCOS) has attracted considerable attention. However, the effect of the dietary inflammatory load remains unclear. Objective. This study was conducted to evaluate the relationship between dietary inflammatory index (DII) score and serum chemerin concentration, biochemical hyperandrogenism and insulin resistance in women with PCOS. Methods. This study, which is a case-control study, was conducted on 44 women with PCOS and 44 healthy women who applied to Ankara Training and Research Hospital. Routine blood biochemical values were obtained from the hospital system and blood samples were taken for serum chemerin analysis. The questionnaire investigated the general information, physical activity status, and anthropometric measurements. The DII was calculated from three-day dietary records Results. The mean DII score of the in the PCOS and control groups is 3.8 ± 1.7 and 3.8 ± 1.4, respectively (p> 0.05). Serum chemerin concentration (3.6 ± 1.4 ng / mL) of the women in the PCOS group with a high DII score was significantly higher than the women in the control group (2.6 ± 1.7 ng / mL) (p <0.05). There was no statistically significant difference between DII score and insulin resistance, total and free testosterone, DHEA-S, LH, FSH, LH/FSH ratio and estradiol values (p>0.05). Conclusions. The highest serum chemerin concentrations was found in the PCOS group with the highest DII. Results suggest that an inflammatory diet may be associated with serum chemerin concentration in PCOS. -
General Endocrinology
Ahmadi R, Oryan S
Effects of estradiol or progesterone on body weight and insulin sensitivity in ratsActa Endo (Buc) 2009 5(3): 299-308 doi: 10.4183/aeb.2009.299
AbstractFemale sex steroids play considerable roles in body weight and insulin physiology.\r\nEnhanced or reduced female sex steroids affect insulin sensitivity.\r\nThe aim of the present study was to examine the effects of female sex steroids on body\r\nweight and insulin sensitivity through ovariectomy and progesterone or estradiol\r\nadministration in rats.\r\nMaterials and Methods. 7 week old female albino (Wistar) rats were used in our study.\r\nAnimals were randomly divided into control, uni-ovariectomised, bi-ovariectomised, sham,\r\nvehicle receiving sham and vehicle or hormone receiving female groups. Progesterone (20\r\nmg/kg/day) or estradiol valerate (200 μg/kg/day) were injected subcutaneously, starting on the\r\nthird day after surgery and continued at daily intervals. After 4 weeks, animals were measured\r\nfor body weight and killed. Following serum collection, fasting serum insulin and glucose were\r\nmeasured and fasting glucose to insulin ratio was considered as index of insulin sensitivity\r\nwhich were compared statistically between the groups.\r\nThe results showed increased insulin sensitivity (glucose to insulin ratio) (IS) and body\r\nweight (BW) in both bi-ovariectomised (bi-ovx) (IS=14.76, BW=237.40 g) and uniovariectomised\r\n(IS=11.33, BW=225.53) rats compared with the control group (IS=9.36,\r\nBW=205.32) (p<0.01). Progesterone or estradiol replacement in bi-ovx rats was followed by\r\nincreased or decreased body weight (264.50 or 205.10) and increased or decreased insulin\r\nsensitivity (20.38 or 8.50) compared with bi-ovx rats, respectively (p<0.05). In nonovariectomised\r\nrats, administration of progesterone resulted in increased and of estradiol in\r\ndecreased body weight (220.6 g and 185.35 g) and insulin sensitivity (18.36 and 5.35)\r\ncompared with control animals (p<0.01).\r\nConclusively, our findings indicate that progesterone is enhancer and estradiol is reducer\r\nof insulin sensitivity in rats. In addition, weight gain after ovariectomy or progesterone\r\ntreatment and weight loss following estradiol treatment did not probably contribute in acting on\r\ninsulin sensitivity. -
Endocrine Care
Zosin I, Bottermann P, Golea O
Cross sectional data in renal osteodystrophyActa Endo (Buc) 2005 1(3): 299-310 doi: 10.4183/aeb.2005.299
AbstractRenal osteodystrophy (RO) encompasses the full range of disorders of mineral metabolism that affects the skeleton in patients with chronic renal failure (CRF). The present study tries to analyze some clinical and biochemical features of RO in a group of cases presenting CRF in hemodialysis program. The study group included 45 cases with different nephropathies. The patients were in a longstanding hemodialysis program (mean period 7.46 ? 8.9 yrs). The cases were divided into three subgroups in relation with the length of dialysis time. The performed determinations comprised: a profile of phospho-calcium metabolism, calciotropic hormones (25-hydroxyvitamin D ? 25 (OH) D3; 1, 25 dihydroxyvitamin D ? 1, 25 (OH)2 D3; serum intact PTH) and serum osseous alkaline phosphatase. Paraclinical investigations were represented by X ray examination of bone and joints (certain sites) and bone mineral density measurements by double energy X-ray absorptiometry (DXA) method. The clinical symptoms and signs of RO were represented by: bone pains, height loss, fractures and acute arthritis. Biochemical assessment showed marked alteration of phosphocalcium metabolism and of the levels of calciotropic hormones, related to the stage of CRF and length of hemodialysis.\r\nThe radiographic aspects displayed different patterns, while DXA revealed in most of studied cases different degrees of bone loss, related to end-stage renal disease and associated factors. -
Notes & Comments
Dutta D, Kumar M, Sen A, Chowdhury J.R, Mukhopadhyay M, Mukhopadhyay S, Chowdhury S
Skull Metastasis as the Presenting Feature of Mixed Medullary and Follicular Thyroid CarcinomaActa Endo (Buc) 2014 10(2): 299-306 doi: 10.4183/aeb.2014.299
AbstractBackground. Skull metastasis has not been reported from mixed medullary follicular thyroid carcinoma (MMFTC). Objective. To present a patient with expansile lytic skull metastasis. Case report. A 61 year lady is presented with goiter for 7 years and 8 cm diameter painful swelling over frontal bone for 18 months, aspiration from which revealed sheets and clusters of polygonal cells, similar to aspiration from hypoechoic nodule in right thyroid lobe. Serum calcitonin (569pg/mL) and carcinoembryonic antigen (11.2ng/mL) were elevated. Histopathology of 3.8×3.1cm nodule in thyroidectomy specimen revealed irregular islands of small polygonal tumor cells with extracellular amyloid deposits (suggesting medullary thyroid carcinoma (MTC)), intermingled with thyroid follicular cells showing capsular and vascular invasion (follicular thyroid carcinoma (FTC)). Immunohistochemistry of the thyroid tumor was negative for calcitonin and for thyroglobulin. Post-operative serum calcitonin and stimulated thyroglobulin were respectively 97 pg/mL and 11.5 ng/mL. I131 whole body scan revealed intense uptake in region of the skull metastasis with small uptake in thyroid bed. She received 150 mCi of I131with resolution of pain, heaviness, throbbing, reduction in swelling size, and lack of disease progression. Conclusions. Skull metastasis was the presenting feature of MMFTC which improved with I131 therapy. Patients with lytic skull metastasis should be evaluated for occult thyroid malignancy. -
General Endocrinology
Korkmaz HA, Karaarslan U, Eraslan C, Atila D, Hazan F, Barisik V, Ata ES, Etlik O, Yildiz M, Ozkan B
Screening of PROP-1, LHX2 and POU1F1 Mutations in Patients with Ectopic Posterior Pituitary GlandActa Endo (Buc) 2018 14(3): 300-306 doi: 10.4183/aeb.2018.300
AbstractObjective. Ectopic posterior pituitary gland (EPP) is usually characterized by an abnormal pituitary stalk and hypoplasia of the anterior hypophysis. The genetic mechanisms involved in the development of EPP remain uncertain. The aim of this study is to determine whether mutations in the three genes, PROP-1, LHX2, and POU1F1, are associated with the risk for and the characteristics of EPP. Methods. In the Endocrinology Outpatient Clinic of “Dr. Behcet Uz” Children’s Hospital, 27 patients with EPP were submitted to sequencing analyses of the PROP-1, LHX2, and POU1F1 genes. Results. Growth hormone, thyrotropin, corticotropin, gonadotropin, and vasopressin deficiency were observed in 22 (81.5%), 23 (85.2%), 17 (63%), 14 (51.9%), and two (7.4%) patients. Thirteen patients (48.1%) presented with hyperprolactinemia. Fourteen patients (51%) had a history of birth dystocia, and 12 cases (42.1%) had a history of breech presentation. Central nervous system abnormalities included five cases with corpus callosum agenesis, one case with schizencephaly, and one case with Chiari type 1 malformation. We identified a homozygous p.S109* mutation in exon 2 in one male patient with EPP and two different PROP1 gene polymorphisms (A142T or c.109+3 G>A polymorphism) in thirteen patients. Conclusions. Our results suggest that PROP1 gene abnormalities might explain the genetic mechanisms involved in the development of EPP. -
General Endocrinology
Sobu E, Yildiz Z, Karaaslan A, Cetin C, Berk Akbeyaz B, Kaya Ozcora GD, Yilmaz B
Evaluation of Fetuin-A Levels in the Early Stage of Autoimmune ThyroiditisActa Endo (Buc) 2023 19(3): 301-306 doi: 10.4183/aeb.2023.301
AbstractContext. Fetuin-A is a multifunctional protein and is known to be related to metabolic syndrome, vascular calcification, and inflammation. Objective. The purpose of this study was to determine the effects of serum fetuin-A levels on autoimmune thyroiditis without thyroid dysfunction. Subjects and Methods. This prospective casecontrol study was performed at the pediatric endocrinology outpatient clinic of a tertiary health institution in Istanbul, Turkey between July 2022 and October 2022. Serum fetuin-A levels were assessed using a human fetuin-A enzyme-linked immunosorbent assay (ELISA) kit (Elabscience Biotechnology, Houston, TX, USA). Results. The study included 86 participants, of which 42 were patients with Hashimoto’s thyroiditis (HT) and 44 were controls. Autoimmune thyroiditis without thyroid dysfunction was found to be related to lower plasma fetuin-A levels. There were no statistically significant differences in the neutrophil-to-lymphocyte ratio, fasting blood glucose level, insulin level, or HOMA-IR (Homeostatic Model Assessment for Insulin Resistance) value between the groups. A fetuin-A level of ≤162.22 μg/mL (80.95% sensitivity and 70.45% specificity) was found to support the identification of autoimmune thyroiditis. Conclusions. The findings of our study suggest that autoimmune thyroiditis without thyroid dysfunction is related to lower fetuin-A levels. Low fetuin-A levels are known to be associated with an increased risk of cardiovascular disease, suggesting that careful monitoring is required in patients with low fetuin-A levels. -
General Endocrinology
Demirel C, Korkmaz H, Gurgul S, Yildiz A, Akarsu E, Erdal N
The Effect of Glucovance Therapy on Biomechanical Deterioration of Bone in Streptozotocin-Induced Diabetic RatsActa Endo (Buc) 2019 15(3): 301-304 doi: 10.4183/aeb.2019.301
AbstractAim. Is to evaluate the influence of glucovance therapy on biomechanical properties of bone in streptozotocin - induced diabetes mellitus (DM) in rats. Materials and Methods. A total of 28 male Wistar- Albino rats (12-week-old; 210-300 g) were divided into 4 groups including control (C; no treatment; n=7), sham [Sh; distilled water (gavage, for 8 weeks); n=7], diabetes [DM; streptozotocin (45 mg/kg, single i.p injection); n=7] and diabetes+ Glucovance treatment [DM+G; streptozotocin (45 mg/kg, single i.p injection) + Glucovance (Glucovance, 500/5 mg/kg/day/rat, gavage, for 8 weeks); n=7] groups. Body weight, blood glucose levels (BGLs), bone mineral density (BMD) and geometric/mechanical properties of bone tissue were evaluated. BGLs in diabetic rats were significantly increased compared to non-diabetic rats, while the body weights were decreased (p<0.05). Results. A significant difference was not detected between groups with regard to cross-sectional area of diaphyseal femur (p>0.05). Maximum load, energy absorption capacity, ultimate stress, ultimate strain, toughness and displacement were shown to decrease and stiffness was shown to increase in DM rats (p<0.05). Ultimate stress and maximum load were significantly increased in DM+G groups compared to DM groups (p<0.05). Conclusion. Glucovance treatment seems to be effective in restoration of biomechanical deterioration of bone specific to STZ-induced DM.