The International Journal of Romanian Society of Endocrinology / Registered in 1938

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  • General Endocrinology

    Guja C, Dumitrascu A, Boscaiu V, Baciu A, Debretin M, Pavel A

    Choroid plexus - pineal gland correlations. Medical anthropology - computed tomography studies. Intracranial physiological calcification

    Acta Endo (Buc) 2005 1(1): 1-18 doi: 10.4183/aeb.2005.1

    Abstract References
    OBJECTIVES: This study was carried out on 1290 patients, whose choroids plexus and pineal gland were examined on computed tomography. Aim: To check the correspondence between the choroid plexuses and the pineal gland calcifications along age groups and sex; and the connections between these calcifications and associated pathology.\r\nMATERIALS AND METHODS: The study group consisted of patients of both sexes, within six age intervals.\r\nRESULTS: In order to classify the calcification variants, eight types of combinations were ordered and can be seen in CT: two refer to extreme variants: totally uncalcified (type 1) and totally calcified (type 8); bilateral, symmetrical variants (types 4 and 5); the other four types include the asymmetrical calcifications (2, 3, 6 and 7). After the anthropological study the results demonstrate that there are significant differences between calcification of the choroids plexus and those of the pineal gland with the two sexes, on age groups and pathological ground. For type 1-totally uncalcified the maximum frequency is around 70% with ages under 19. For type 8 - totally calcified, bilateral, the maximum frequency is around 50% with age groups 48-59 and 60-71. For type 4 - calcification only of choroid plexus, one finds a continuous increase from about 10% at the first age group to about 25% at the last group, while for type 5- calcification only of the pineal gland the frequency is 10%−20%. We started from the hypothesis that the presence of these calcifications is physiological, and has an active adaptative metabolic part depending on many factors, among which the individual constitutional ground is also present.\r\nCONCLUSIONS: The age is not the main cause of the calcification types, but a process of adaptative-reactive variability of interface type, playing an integrating mediating part.
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    8. Danaila L, Golu M, Tratat de neuropsihologie, Vol. I. Bucuresti: Editura Medicala, 2000: 526−541.
    9. Zhang S, Janciauskiene S. Multi-functional capability of proteins: α1-antichymotrypsin and the correlation with Alzheimer?s disease. Journal of Alzheimer?s Disease 2002; 4(2): 115.
    10. Anthony SG, Schipper HM, Tavares R, Hovanesian V, Cortez SC, Stopa EG, Johanson CE. Stress Protein Expression in the Alzheimer-Diseased Choroid Plexus.. Journal of Alzheimer?s Disease 2003; 5(3):171.
    11. Kodaka T, Mori R, Debari K, Yamada M. Scanning Electron Microscopy and Electron Probe Microanalysis Studies of Human Pineal Concretions, J. Electron Microsc 43(5): 307-317.
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    16. Coculescu M, Humoral Markers of Neuroendocrine Tumors: Utility in Diagnosis and Therapy, Abstrat Book - 2nd Regional ISPNE Congress, June 6-8, 2002, Parliament Palace, Bucharest.
  • General Endocrinology

    Badiu C, Dumbrava M, Stancu C, Ciubotaru V, Coculescu M

    Immunoreactivity for glycoproteic hormones and tumor size in pituitary adenomas

    Acta Endo (Buc) 2006 2(1): 1-9 doi: 10.4183/aeb.2006.1

    At least one fifth of pituitary adenomas exhibit plurihormonality when using immunohistochemistry for anterior pituitary hormones. However, the correlation with clinical features is weak, without an agreement upon pathological predictors of tumor behavior. The aim was to determine the immunoreactivity for anterior pituitary hormones and alpha subunit in 276 consecutive pituitary adenomas patients, aged 22-79 years (44.3 ? 8), 154 F/ 122 M: 83 acromegalics (ACM), 173 nonfunctioning adenomas (NFA) and 20 prolactinomas (PRM) submitted to surgery via transfrontal (81) or transsphenoidal (195) along 10 years (1995-2005). In addition, clinical data, hormonal secretion and tumour size were evaluated before pituitary surgery. Local ethical committee approved the study design. The immunoreactivity performed by the avidin-biotin-complex method was evaluated for beta FSH, LH, TSH, alpha subunit, PRL and GH, using a semiquantitative scale of stained cells: strong (>20%), positive (10-20%), weak (5-10%) and negative (<5%). CT or MRI tumor size (less than 1 cm, 1-2 cm, 2-4 cm and over 4 cm on maximal diameter) were considered together with the Hardy neuroradiological stage. The results showed that 16/83 ACM, 53/173 NFA and 4/20 PRM exhibited immunoreactivity for beta FSH and LH. TSH immunoreactivity was positive in 13/83 ACM, 11/173 NFA and 1/20 PRM. Tumor size in gonadotrophin - positive group (> 10% of stained cells) was between 1-2 cm in 6 ACM, 21 NFA and 2 PRM, while positive bigger tumors (2-4 cm) were in 7 ACM, 24 NFA and 2 PRM. Giant, over 4 cm tumors were positive in 3 ACM, 8 NFA and no PRM. A similar trend of the tumor size distribution was observed in the monohormonal or null cell adenomas. In conclusion, tumor size and gonadotrophin plurihormonality are independent factors in the management of pituitary adenomas.
  • General Endocrinology

    Leonte L, Coculescu M, Radian S, Fica S, Caragheorgheopol A, Marinescu B, Bohaltea LC, Grigorescu F

    Anti-Mullerian hormone (AMH) as a useful marker in diagnosis of polycystic ovary syndrome

    Acta Endo (Buc) 2007 3(1): 1-12 doi: 10.4183/aeb.2007.1

    The mechanism underlying anovulation in the polycystic ovary syndrome (PCOS) remains unclear, although an excessive number of small antral follicles at ultrasound scans and discrepancies with selected follicles sustain the hypothesis of altered follicular development. Anti-M?llerian (AMH) hormone is a member of TGF-b super family of growth factors produced by granulosa cells of pre- and small-antral follicle. The 2 to 3 fold increase in the number of growing follicles in the ovary from PCOS women is reflected by an increase in serum concentration of AMH and thus, this hormone may be a good marker of PCOS.\r\nAim. This study was intended to implement ultra-sensitive ELISA measurement of serum AMH from PCOS women and search for a potential correlation with clinical and laboratory parameters.\r\nSubjects and methods. Sera from patients with PCOS (n = 42) and control women (n = 22) were used for ELISA measurement of AMH (AMH-EIA, Beckman Coulter) with sensitivity of 0.7 pmol/L.\r\nResults. We found a serum concentration of AMH almost 3 folds higher in patients with PCOS compared to controls (73.7 ? 7.5 vs. 25.7 ? 3.9 pmol/L, P < 0.0001). Differences were even higher in lean subjects. A positive correlation was found between total testosterone and LH levels, but not with serum FSH or insulin. Moreover, AMH concentration was correlated to more hyperandrogenic PCOS and with amenorrhea, and thus to the severity of the syndrome.\r\nConclusion. Measurement of serum AMH may be used as a valuable marker for PCOS to confirm diagnosis and evaluate the extent of follicular dysfunction in relation with hyperandrogenism and menstrual disturbances.
  • General Endocrinology

    Lutescu I, Gussi I, Banceanu G, Coculescu M

    Glycoproteic hormones, thyroxine, estradiol and prolactin profiles across the blood cerebro spinal fluid (CSF) barrier in pregnancy

    Acta Endo (Buc) 2008 4(1): 1-9 doi: 10.4183/aeb.2008.1

    The blood-CSF barrier (BCB), as a component of the blood-brain barrier, is protective for the maternal brain. This study assesses estradiol, prolactin, glycoproteic hormones (hCG, FSH, LH, TSH) and thyroxine across the BCB in pregnancy after 38 weeks. Method. 35 pregnant women were simultaneously sampled in serum and CSF during caesarian section and compared to 27 non-pregnant fertile women undergoing surgery for benign gynecological disorders. The study was approved by the local Ethics Committee. Results were analysed as nonparametric variables. Compared to non-pregnant controls, we found high serum estradiol levels at term, also reflected in the CSF, while the CSF/serum ratio was non-significantly modified (median ratio 0.1 versus 0.1, p=NS). Prolactin showed a similar proportional increase in serum and CSF levels at term, with unmodified CSF/serum ratio (median ratio 0.14 versus 0.18, p=NS). hCG showed a similar profile across the BCB. FSH was significantly lower at term, but still conserved the CSF/ serum ratio. LH was undetectable in pregnancy. In peripartum TSH showed a unique profile across the BCB as it was the only one showing an increased CSF/serum ratio compared to non-pregnant controls (median ratio 0.11 versus 0.04, p<0.0001). Thyroxine was significantly increased in both serum and CSF, and showed a CSF/serum ratio unmodified from non-pregnant women (median ratio 0.02 versus 0.02, p=NS). Conclusion. There is an increase of BCB permeablity for TSH in term pregnancy. The peripartum increase in estradiol and decrease in HCG could be involved. We suggest that the unique TSH profile maintains the necessary thyroxine levels in pregnancy at term.
  • General Endocrinology

    Pascu A, Radoi M, Coculescu M

    Limits in using brain natriuretic peptide (BNP) as a biomarker of acute right ventricular dysfunction in pulmonary embolism

    Acta Endo (Buc) 2009 5(1): 1-18 doi: 10.4183/aeb.2009.1

    Background. Risk stratification and an appropriate therapeutic approach could be lifesaving in acute pulmonary embolism (PE). Echocardiographic (ECHO) acute right ventricular dysfunction (RVD) is the actual &#8220;gold standard&#8221; in risk evaluation of PE. We previously demonstrated that plasma BNP levels were significantly higher in patients with PE and acute RVD on ECHO vs. patients with normal RV function on ECHO.\r\nAim. Evaluation of the limits of plasma BNP in signalling acute RVD in PE. \r\nMethods. 70 patients with PE were prospectively investigated: 42(60.0%) men, mean ? SD(standard deviation) age 52.51?8.82. BNP was measured on admission using a quantitative fluorescence immunoassay (TriageBNP). ECHO evaluation of the RV function was performed in the first hour after admission. Study protocol was approved by local Ethical Committee. Patients were divided into two groups: group 1-with acute RVD on ECHO, n=24(34.3%) patients; group 2 - without acute RVD on ECHO, n=46(65.7%).Patients from group 1 were further divided into two subgroups: subgroup 1A-admitted in <12 hours after their PE symptoms onset, n=12(50.0%) patients, and subgroup 1B-admitted in >12 hours after the onset of PE symptoms, n=12(50.0%) patients.\r\nResults. BNP proved good in discriminating between patients with and without acute RVD (AUC=0.88, P<0.0001). The cut-off level of plasma BNP=50 pg/mL showed the best sensitivity=0.86 and specificity=0.82 in identifying acute RVD. BNP levels were significantly lower in subgroup 1A (admitted soon) compared to subgroup 1B (admitted later than 12 hours): medians 45.25 pg/mL vs. 344.50 pg/mL, P<0.0001. Eight patients from subgroup 1A, all admitted soon after the onset of their PE symptoms, and all experiencing at least one syncopal episode showed BNP under the cut-off level. In subgroup 1A BNP did not correlate with RV end-diastolic diameter (R=0.23, P=NS), while in subgroup 1B BNP and RV end-diastolic diameter showed a consistent positive correlation (R=0.91, P<0.0001). In subgroup 1A BNP correlated significantly, but negatively, with RV systolic pressure (R=-0.64, P<0.01). In subgroup 1B BNP was significantly positively correlated with RV systolic pressure (R=0.76, P<0.001).\r\nConclusions. BNP higher than a cut-off level of 50 pg/mL could predict acute RVD in patients with PE with a good sensitivity and specificity. Exception of this rule was found in some patients with recent (<12 hours) PE symptoms onset and poor clinical condition.
  • General Endocrinology

    Bandyopadhyay R, Chakraborty S

    Adrenal corticoids induce pineal gland stimulation associated with adrenocortical inhibition of karyomorphology cell proliferation and hormone milieu in male mice (mus musculus)

    Acta Endo (Buc) 2010 6(1): 1-14 doi: 10.4183/aeb.2010.1

    Background. Pineal and adrenocortical cell morphology, dynamics, hormonal analysis and function in response to both natural and synthetic corticoids awaits in depth\r\ninvestigation in mammals.\r\nAim. To investigate the pineal responsiveness to corticoid treatment from combined morphological and hormonal studies in postpubertal male mice.\r\nMaterial and methods. Three groups, each with 14 mice were used as control (C) or treated with the natural corticoid, hydrocortisone (HYC) at a dose of 4 mg/100 g.b.w. and\r\nsynthetic corticoid, dexamethasone (DEX) at a dose of 4 mg/100 g.b.w. for ten consecutive days.\r\nResults. The treatment induced inverse changes in pineal-adrenocortical karyomorphology, cell proliferation (mitotic percentage M%) and hormonal milieu. Whereas both these corticoids caused pineal stimulation as evidenced from significantly increased nuclear diameter (&#956;m) values (C 3.35 ? 0.05, HYC 4.77 ? 0.02, DEX 4.59 ? 0.04, p<0.001) and cell proliferation (M%) (C 1.11 ? 0.09, HYC 1.59 ? 0.07, DEX 1.44 ? 0.05, p<0.01), the changes induced in adrenocortical nuclear diameter in all the zones (p<0.001),\r\ncell proliferation (M%) (C 1.38 ? 0.05, HYC 0.53 ? 0.06, DEX 0.70 ? 0.05, p<0.001) anddecreased content of adrenal corticosterone (C 0.24 ? 0.03, HYC 0.13 ? 0.01, p<0.001 DEX\r\n0.15 ? 0.02, p<0.01) were those of adrenocortical inhibition.\r\nConclusion. There exists an inverse relationship between the pineal and adrenocortical functions in post pubertal male mice (Mus musculus).
  • General Endocrinology

    Capatina CA, Caragheorgheopol A, Marzan L, Toma E, Gandea C, Constantinoiu S, Coculescu M

    Pituitary Hormones in Human Cerebrospinal Fluid

    Acta Endo (Buc) 2011 7(1): 1-10 doi: 10.4183/aeb.2011.1

    Introduction. The blood brain barrier (BBB) restricts the transport of hydrophilic molecules such as peptidic pituitary hormones into the brain tissue. The blood-cerebrospinal fluid (CSF) is a part of the BBB.\r\nAim To compare the pituitary hormone levels on the two sides of the BBB in a group of subjects without endocrine diseases.\r\nPatients and methods. We investigated, with the approval of the local ethics committee, 78 subjects without endocrine diseases. Growth hormone (GH), prolactin (PRL), follicle-stimulating hormone (FSH), luteinizing hormone (LH) and thyroid stimulating hormone (TSH) were measured by rapid fluoroimmunoassay with Europium in the blood and cerebrospinal fluid (CSF)sampled simultaneously before rachianestesia for minor surgery.\r\nResults. CSF concentrations are significantly lower than the corresponding serum ones for all hormones studied: 0.04 ? 0.009 mU/mL vs 2.29 ? 0.57 mU/mL for GH, 1.49 ? 0.078 ng/mL vs 10.07 ? 1.42 ng/mL for PRL, 0.57 ? 0.078 U/L vs 22.71 ? 3.65 U/L for FSH, 0.39 ? 0.038 U/L vs 11.11 ? 1.55 U/L for LH and 0.01 ? 0.003 &#956;U/mL vs 1.36 ? 0.17&#956;U/mL for TSH (mean ? SEM; p<0.001). The CSF/serum ratio was below 1 in the vast majority of cases (from all subjects studied we only found 3 cases with supraunitary CSF/serum ratio). The serum and CSF levels were not significantly correlated for\r\nany of the pituitary hormones. Comparing preand postmenopausal women the CSF gonadotropin levels were slightly but nonsignificantly increased after menopause,\r\ndespite marked differences in the serum concentrations: CSF FSH 1.21 ?0.17U/L after vs 0.84? 0.4U/L before menopause, CSF LH 0.60? 0.047U/L after vs 0.43? 0.14U/L before\r\nmenopause. The CSF/ serum ratio for FSH markedly decreased after menopause (0.02?0.003 vs 0.22?0.11) although the effect did not reach statistical significance. The same\r\nwas true for CSF/serum LH ratio (0.026?0.005 vs 0.09?0.002). For none of the hormones studied the CSF levels correlated with age.\r\nConclusion. Pituitary hormones are normally found in the CSF at much lower levels than in the serum. The CSF hormonal\r\nconcentrations do not significantly correlate with the serum ones.
  • Perspectives

    Gheorghiu ML

    Requirements in Order to Become an European Endocrinologist

    Acta Endo (Buc) 2012 8(1): 1-3 doi: 10.4183/aeb.2012.1

  • Perspectives

    Prada G

    European Call for Longevity. New Perspectives Upon Ageing

    Acta Endo (Buc) 2013 9(1): 1-10 doi: 10.4183/aeb.2013.1

    Over the last decades aging has become a major concern for both medical professionals and policy makers. European Commission has been launching a series of initiatives aimed at drawing attention to great social, political and economic importance of aging research and at finding as well as disseminating the best practices in order to attain a very important objective: to add two years of healthy live to people in Europe by 2020. Nevertheless, there is still controversy regarding the accepted age limit for older people and also concerning various aspects of aging and aging research, most important being the difficulty of correlating chronological age to biological age. A new perspective is suggested and this might help scientists and clinicians understand better the problems of old age and could facilitate targeting research and medical strategies to the most appropriate goals. Several very important projects in the field of aging, funded by European Commission, are presented and a novel approach to the specific situation of older people is offered.
  • Perspectives

    Benga G


    Acta Endo (Buc) 2014 10(1): 1-8 doi: 10.4183/aeb.2014.1

    The water channels milestones include: the vague idea of “hydrophilic pores” or “water-filled channels” in the red blood cell (RBC), the proposal that water channels (WCh) are accommodated in proteins, experiments associating WCh with the major RBC membrane protein called Band 3 (the anion exchanger), and the crucial experiment performed in 1985 by Benga group in Cluj-Napoca, Romania, proving the presence and location of a minor protein of the RBC membrane involved in water transport. In the landmark papers of 1986, Benga introduced the concept of the WCh being a protein specialized in water transport, i.e. a water channel protein (WCP). The first WCP discovered by our group was re-discovered in 1992 by Agre group. In the same year two other WCPs were discovered. The name aquaporins was proposed in 1993. In subsequent years hundreds of WCPS have been discovered in organisms from all kingdoms of life. WCPs are a family of membrane proteins, belonging to the Membrane Intrinsic Proteins superfamily. WCPs family include three subfamilies: 1) aquaporins (AQPs) which are mainly water selective channels; 2) aquaglyceroporins are permeable to water and to other small uncharged molecules; 3) S-aquaporins (subcellular or superaquaporins). Benga called aquaglyceroporins and S-aquaporins the “relatives of aquaporins”. Twelve WCPs were identified in the human body, having a great importance in a lot of physiological phenomena, as well as in pathological conditions, from well defined “water channelopathies” to a wide range of diseases. Benga propose the name of aquaporinology for the domain of biomedical and natural sciences dedicated to the integrated approach of WCPs (aquaporins and relatives), which is also a chapter of Cellular and Molecular Biology.